Abstract
Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV). The analysis laboratory diagnosis of HIV infection is a crucial aspect of controlling AIDS. The p24 antigen, the HIV-1 capsid protein, is of considerable diagnostic interest because it is detectable several days earlier than host-generated HIV antibodies following HIV exposure. We present herein a new sandwich HIV p24 immunosensor based on directly electroplating an electrode surface with gold nanoparticles using chronoamperometry, which greatly increased the conductivity and reversibility of the electrode. Under optimum conditions, the electrochemical signal showed a linear relationship with the concentration of p24, ranging from 0.01 ng/mL to 100 ng/mL (R > 0.99), and the detection limit was 0.008 ng/mL. Compared with ELISA, this method increased the sensitivity by more than two orders of magnitude (the sensitivity of ELISA for p24 is about 1 ng/mL). This immunosensor may be broadly applied to clinical samples, being distinguished by its ease of use, mild reaction conditions, guaranteed reproducibility, and good anti-interference ability.
Highlights
Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV)
Cyclic voltammetry is a simple and easy method to study the characteristics of the p24 antigen biosensor at its different preparation phases
When the same redox reaction was carried out at the Au/glassy carbon electrode (GCE), the cyclic voltammogram obtained shows a 110% increase in peak current compared to that in trace a. This is attributable to the increase in surface area by the gold nanoparticles on the GCE
Summary
Acquired immune deficiency syndrome (AIDS) is a severe communicable immune deficiency disease caused by the human immune deficiency virus (HIV). HIV is categorized into HIV-1 and HIV-2, which differ largely in their nucleotide sequences. HIV-2 is largely restricted to some regions of Western Africa and HIV-1 is found in most HIV strains worldwide, including China. There are still no efficacious therapeutic measures. Prevention of infection is the primary strategy to control HIV. The analysis laboratory diagnosis of HIV infection is a crucial aspect of controlling AIDS. A sensitive and practical detection method to monitor, diagnose, and screen HIV infection is especially important for controlling AIDS
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
