Abstract
A sandwich enzyme immunoassay for the measurement of human growth hormone in serum was developed using antibody IgG-β-D-galactosidase conjugate and anti-body IgG-coated silicone rubber rods. The sensitivity of the assay was 4pg/tube, which corresponded to 0.4 ng/ml, when 10μ1 of serum was used. The assay system showed little cross-reaction to other human pituitary hormones including prolactin, thyroid-stimulating, follicle-stimulating and luteinizing hormones and human chorionic gonadotropin. The coefficients of variation for different levels of serum hGH ranged from 5.8–9.3% and 6.7–14.5%, for within and between run, respectively. The regression equation and coefficient for correlation to radioimmunoassay were y(EIA) = 1.06 x(RIA) − 0.22 and 0.97 ( n = 46), respectively.
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