Abstract
Infectious diseases, especially pathogenic infections, are a growing threat to public health worldwide. Since pathogenic bacteria usually exist in complex matrices at very low concentrations, the development of technology for rapid, convenient, and biocompatible sample enrichment is essential for sensitive diagnostics. In this study, a cucurbit[6]uril (CB) supermolecular decorated amine-functionalized diatom (DA) composite was fabricated to support efficient sample enrichment and in situ nucleic acid preparation from enriched pathogens and cells. CB was introduced to enhance the rate and effectiveness of pathogen absorption using the CB–DA composite. This novel CB–DA composite achieved a capture efficiency of approximately 90% at an Escherichia coli concentration of 106 CFU/mL within 3 min. Real-time PCR analyses of DNA samples recovered using the CB–DA enrichment system showed a four-fold increase in the early amplification signal strength, and this effective method for capturing nucleic acid might be useful for preparing samples for diagnostic systems.
Highlights
Pathogenic infections result in diseases caused by toxins released by pathogenic organisms, and such infections are a growing threat to human health and public health worldwide [1,2,3].Currently, pathogen identification and therapeutic approaches play important roles in controlling infections
As we reported in our previous study, cucurbituril-based diatom composites (CB–diatomaceous earth (DA)) exhibit a strong host–guest interaction that supports molecular encapsulation [11]
Using scanning electron microscopy (SEM) (JEOL JSM-7500F, Tokyo, Japan) and dynamic light scattering (DLS) (DynaPro NanoStar, Wyatt), we confirmed that the size of the diatomaceous earth (DE) particles was well distributed between 10 and 20 μm—an appropriate size range for this project (Figure 1A,B)
Summary
Huifang Liu 1,2,† , Geun Su Noh 1,2,† , Yange Luan 1,2 , Zhen Qiao 1,2 , Bonhan Koo 1,2 , Yoon Ok Jang 1,2 and Yong Shin 1,2, *. These authors (H.L & G.N) contributed to this work
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