Abstract
Salmonella is a kind of pathogenic bacteria that is widely present in various types of food, and its virulence and source are closely related to its serogroups. In order to rapid and accurate identification of the Salmonella serogroups in an unknown sample, the Salmonella serogroups-RAA high throughput microfluidic (SSG-RAA HTM) system based on real-time recombinase aided amplification (RAA) was established for simultaneous detection of Salmonella genus and five serogroups (B, C1, C2-C3, D, and E). Compared with conventional methods, the microfluidic system has obvious advantages in the detection time (15–40 min) and the reliability of result interpretation. The lowest detection limits of SSG-RAA HTM system were 101 CFU·mL−1 (genus and serogroup B, C1, C2-C3) or 102 CFU·mL−1 (serogroup D, E). The newly developed system has excellent stabilities (C.V.s < 5%) and possessed accuracies (>98%) in the actual sample verification. Therefore, this microfluidic strategy has great application prospects in pathogen assessment.
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