Abstract

In the course of some quantitative studies on the growth of anaerobes in various plant and animal products, one of the authors (C. C. D.) has, during the past year, employed 13 large anaerobic jars. They have been operated in accordance with the principles proposed by Laidlaw 1 and constructed according to the descriptions published by Mcintosh and Fildes.2 It is the purpose of this paper to enumerate briefly the various details and operations which have eliminated the danger of explosions. The arrangement of the equipment as used in this laboratory is shown in fig. 1. It will be seen from the illustration that the jar is not unlike that described by Brown.3 A few alterations in construction are indicated in the photograph and are briefly summarized as follows: (1) The wires conveying current to the heating coil are soldered4 to short pieces of Pyrex glass tubing, which are then cemented into a rubber stopper (A). This method of introducing the lead wires readily permits the use of multiple stranded copper wire (as ordinary, flexible electric light cord), minimizing the chance of breakage. The wires should be looped over and tied to the side of the glass tubes on the outside of the jar top to prevent any strain from occurring at the soldered joint. (2) A shield of asbestos sheet (B) is placed over the upper surface of the coil to prevent the heat from cracking the jar top. (3) Instead of mica as used by Mcintosh and Fildes, asbestos washers are placed at these points (C) to deflect the heat from the inner ends of the rubber stoppers. In several papers,5 it is emphasized that the anaerobic apparatus embodying the principle of Brown's jar produces excellent results, but

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call

Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.