Abstract
AbstractA semi‐micro method is described for the determination by gas‐liquid chromatography (g.l.c.) of galacturonic acid in pectic substances which has been solubilised in aqueous ammonium oxalate. Galacturonic acid, liberated by hydrolysis with polyanhydrogalacturonase [poly‐(1,4‐α‐D‐galacturonide) glycanohydrolase; EC 3.2.1.15] and then converted to the L‐galactono‐1,4‐lactone via potassium borohydride reduction and lactonisation using methanolic hydrogen chloride. The derived lactone was analysed by g.l.c. as the trimethylsilyl derivative, and was easily distinguished from the glucuronic acid reduction product, L‐gulono‐1,4‐lactone. The method was applied to the analysis of pectic materials in leaflets, petioles, and stem of three tropical legumes, and also to the polygalacturonic content of commercial pectin preparations. The possibility of simultaneous determination of starch extracted with pectin is also discussed.
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