A role for endoplasmic reticulum exit sites in foot-and-mouth disease virus infection

Rebecca Midgley,Daniel Fullen,David J Stephens,Katy Moffat,Philippa Hawes,Stephen Berryman,Jennifer Simpson,Alison Burman,Terry Jackson

doi:10.1099/vir.0.055442-0

Rebecca Midgley, Daniel Fullen + Show 7 more

Open Access

https://doi.org/10.1099/vir.0.055442-0

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Abstract

Picornaviruses replicate their genomes in association with cellular membranes. While enteroviruses are believed to utilize membranes of the early secretory pathway, the origin of the membranes used by foot-and-mouth disease virus (FMDV) for replication are unknown. Secretory-vesicle traffic through the early secretory pathway is mediated by the sequential acquisition of two distinct membrane coat complexes, COPII and COPI, and requires the coordinated actions of Sar1, Arf1 and Rab proteins. Sar1 is essential for generating COPII vesicles at endoplasmic reticulum (ER) exit sites (ERESs), while Arf1 and Rab1 are required for subsequent vesicle transport by COPI vesicles. In the present study, we have provided evidence that FMDV requires pre-Golgi membranes of the early secretory pathway for infection. Small interfering RNA depletion of Sar1 or expression of a dominant-negative (DN) mutant of Sar1a inhibited FMDV infection. In contrast, a dominant-active mutant of Sar1a, which allowed COPII vesicle formation but inhibited the secretory pathway by stabilizing COPII coats, caused major disruption to the ER–Golgi intermediate compartment (ERGIC) but did not inhibit infection. Treatment of cells with brefeldin A, or expression of DN mutants of Arf1 and Rab1a, disrupted the Golgi and enhanced FMDV infection. These results show that reagents that block the early secretory pathway at ERESs have an inhibitory effect on FMDV infection, while reagents that block the early secretory pathway immediately after ER exit but before the ERGIC and Golgi make infection more favourable. Together, these observations argue for a role for Sar1 in FMDV infection and that initial virus replication takes place on membranes that are formed at ERESs.

Highlights

Foot-and-mouth disease (FMD) is one of the most economically important viral diseases of domestic livestock affecting cattle, sheep, goats and pigs (Scudamore & Harris, 2002)
Sar1 is converted to its inactive GDP-bound form and COPII coats rapidly dissociate from the vesicles (Stephens et al, 2000), which acquire COPI in a process known as COPII/COPI exchange before fusion with the ER–Golgi intermediate compartment (ERGIC)
GBF1 is the only known ADP-ribosylation factor 1 (Arf1)–guaninenucleotide exchange factor (GEF) localized to the cis-Golgi and is required for transport-vesicle trafficking between the endoplasmic reticulum (ER) and Golgi (Alvarez et al, 2003; Claude et al, 1999; Kawamoto et al, 2002), whereas BIGs are responsible for Arf1 recruitment on the trans-Golgi (Manolea et al, 2008)

Summary

Introduction

Foot-and-mouth disease (FMD) is one of the most economically important viral diseases of domestic livestock affecting cattle, sheep, goats and pigs (Scudamore & Harris, 2002). The above results confirmed that BFA disrupts the ERGIC and Golgi and showed that FMDV infection does not require these organelles to be intact.

Results

Conclusion