Abstract
A Role for Biotin in Yeast Glycolysis
Highlights
Biotin stimulates the fermentation of glucose and fructose by air-dried yeast grown deficient in this vitamin
Glucose-6-phosphate dehydrogenase can be measured in airdried yeast
freshly harvested deficient yeast can be stimulated by the addition of free biotin
Summary
More recently [3], it has been demonstrated that the hexokinase activity of cell-free extracts from biotin-deficient yeast is much more diminished than that of extracts from cells grown under optimal conditions These investigators were unable to stimulate the activity of hexokinase in these preparations by the addition of biotin, and the site of biotin action in carbohydrate fermentation was still undetermined. By the use of manometric and spectrophotometric techniques we have been successful in stimulating the rate of glucose and fructose fermentation and the hexokinase activity of air-dried cells of biotin-deficient yeast. The activity of these dried preparations on the phosphorylated hexoses is not increased in the presence of biotin. Protein determinations in cell-free extracts were made by the method of Stadtman et al [8]
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