Abstract
Glial cells such as astrocytes and microglia are critical regulators of inflammation after spinal cord injury (SCI). Existing in vitro studies of glial cells do not account for the complexity of this inflammatory cascade. Therefore, there is a critical need to develop a standardised model which replicates the inflammatory phases associated with SCI. Here, we report an in vitro model of mixed glial culture (MGC) where inflammation is induced by the administration of injury specific pro-inflammatory cytokines (tumour necrosis factor-α (TNF-α), interleukin (IL)-1β and interleukin (IL)-6) to promote pathologically relevant “acute-one day” and “chronic-21 days” inflammatory phases. We observed SCI relevant differential modulation of nuclear factor kappa-light-chain-enhancer of activated B cells (NFκB)-p65 and mitogen-activated protein kinase (MAPK)-p38 pathways and associated cytokines, chemokines and growth factors over 21 days.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
