Abstract
A unique fluorescent probe, MCB, for HClO/ClO- was rationally constructed by integrating methylene blue, 7-hydroxycoumarin, and two HClO-specific reacting moieties into a single molecular structure. Distinct from other published cases, MCB displayed remarkable dual-channel fluorescence enhancement, peaked at 464 and 700 nm respectively, which simultaneously provided two crosstalk-free fluorescence channels, blue and NIR channels, for mutual corroboration, and hence improving the reliability of the detection results. More importantly, MCB exhibited excellent sensing properties for HClO/ClO-, such as fast response (within 90 s), high sensitivity (LOD as low as 3.9 nM based on blue fluorescence channel), and sufficient selectivity. Significantly, MCB was successfully utilized for dual-channel imaging of HClO/ClO-in living cells and zebrafish, thereby holding the potential for research that focused on elucidating the biological roles of HClO/ClO-.
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