Abstract
A region of bacteriophage lambda P R RNA which binds efficiently to Escherichia coli ribosomes has been isolated from the 5′ end of an in vitro transcript. Analysis of the 45 nucleotides protected by ribosomes from nuclease digestion reveals a central AUG triplet and a stretch of nine bases which are complementary to the 3′ end of E. coli 16 S ribosomal RNA. The nine base complementarity is the longest among known initiator regions from E. coli and bacteriophage messenger RNAs. The existence of a strong ribosome binding site at the 5′ end of P R RNA potentially locates the translation initiation site for the λ cro gene product. The nucleotide sequence beyond the AUG initiation codon predicts an N-terminal amino acid sequence for the Cro protein of (Met)-Glu-Gln-Arg-Ile-Thr-Leu-Lys-Asp.
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