A review on in-vitro oral bioaccessibility of organic pollutants and its application in human exposure assessment

Abstract

Generally, human oral exposure assessments of contaminants have not considered the absorption factor in the human gastrointestinal tract, thus overestimating human exposure and associated health risk. Currently, more researchers are adding the absorption factor into human exposure assessment, and bioaccessibility measured by in-vitro methods is generally replacing bioavailability for estimation because of the cheap and rapid determination. However, no single unified in-vitro method is used for bioaccessibility measurement of organic pollutants, although several methods have been developed for these pollutants and have shown good in vitro-in vivo correlation between bioaccessibility and bioavailability. The present review has focused on the development of in-vitro methods, validation of these methods through in-vivo assays, determination of factors influencing bioaccessibility, application of bioaccessibility in human exposure assessment, and the challenges faced. Overall, most in-vitro methods were validated using bioavailability, and better in vitro-in vivo correlations were obtained when absorption sinks were added to the digestion solution to mimic dynamic absorption of organic chemicals by small intestine. Incorporating bioaccessibility into the estimation of human exposure by oral ingestion significantly decreases the estimated exposure dose. However, more investigations on bioaccessibility of hydrophobic organic compounds are urgently needed because many challenges for in-vitro methods remain to be overcome.