Abstract
In this study, researches on inteins and recombinant proteins to increase splitting activity of inteins that are utilised in recombinant protein purification processes without use of protease have been investigated. Researches on protein cleaving by use of inteins are aimed to make inteins and protein purification processes become more effective. The processes conducted in vivo ve in vitro, intein and target protein included polyclonal PCR products are cloned and the expressed plasmids are fused to cloned strains. Kinteics of activity of cleaving and activity of cleaving rates have been determined in different pH, temperature and filtration times. In the researches, protein cleaving activity of the different microbial natural and engineered inteins have been investigated and cleaving activity of recombinant Nostoc punctiforme DnaE C-inteins were found to be very high. The studies have been focused on the C-inteins due to recepient ability of the amino acid additions.
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