A review on immobilization strategy of cyclodextrin glucanotransferase producing Escherichia coli as whole cell biocatalyst

Abstract

This paper reviewed the immobilization strategy on cyclodextrin glucanotransferase (CGTase) producing Escherichia coli as whole cell biocatalyst. Biotransformation of insoluble substrate such as starch and lignocellulosic biomass with whole cell leads to the application of biological steps to chemocatalysis, which makes the whole process more effective. In this whole cell biocatalysis process, the excretion of the enzymes into the extracellular space by the cell is crucial. However, the use of free whole cell biocatalysts especially Escherichia coli often face with problem related to the cell lysis and stability. Due to that, the biotransformation process becomes inefficient. Thus, in this study the immobilization strategy was investigated to overcome the bottle necks mentioned. First, this study began with a focus in Escherichia coli, immobilization techniques, cyclodextrin, cyclodextrin glucanotransferase and recombinant Escherichia coli, in which can give a better understanding and a bigger picture in order to overcome the bottle necks mentioned. The different immobilization strategy and condition identified expected to improve the whole cell stability and reusability which lead to efficient biotransformation process. The output of the research also will elucidate the mechanism on how different immobilization material, strategy and cultural conditions improve the overall cell performance.