Abstract
The main downside of utilizing algal biomass for biofuel production is the rigid cell wall which confines the availability of soluble organics to hydrolytic microbes during biofuel conversion. This constraint reduces the biofuel production efficiency of algal biomass. On the other hand, presenting various pretreatment methods before biofuel production affords cell wall disintegration and enhancement in biofuel generation. The potential of pretreatment methods chiefly relies on the extent of biomass liquefaction, energy, and cost demand. In this review, different pretreatments employed to disintegrate algal biomass were conferred in depth with detailed information on their efficiency in enhancing liquefaction and biofuel yield for pilot-scale implementation. Based on this review, it has been concluded that combinative and phase-separated pretreatments provide virtual input in enhancing the biofuel generation based on liquefaction potential, energy, and cost. Future studies should focus on decrement in cost and energy requirement of pretreatment in depth.
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