Abstract
Ultraviolet spectroscopy is one important and advanced analytical instrument in Pharmaceutical industry and used since last 35 years and. The method of analysis is based on measuring the absorption of a monochromatic light by colorless compounds in the near ultraviolet path of spectrum (200-400nm). The pharmaceutical analysis comprises the procedures necessary to determine the “identity, strength, quality and purity” of such compounds. It also includes the analysis of raw material and intermediates during manufacturing process of drugs. The fundamental principle of operation of spectrophotometer covering UV region consists in that light of definite interval of wavelength passes through a cell with solvent and falls on to the photoelectric cell that transforms the radiant energy into electrical energy measured by a galvanometer. Ultraviolet-visible spectroscopy is used to obtain the absorbance spectra of a compound in solution or as a solid.
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