A review of the novel thermophilic Campylobacter, Campylobacter hepaticus, a pathogen of poultry.

Abstract

In 2015, a novel thermophilic Campylobacter was isolated from cases of spotty liver disease in laying hens in the UK. In 2016, it was isolated from laying hens in Australia where it was formally named Campylobacter hepaticus and confirmed as the cause of spotty liver disease. It has also been isolated from laying hens in America. It is fastidious, grows slowly on first isolation and does not grow on media used to routinely isolate Campylobacter. Spotty liver disease is an acute, randomly distributed, focal, necrotic hepatitis causing mortality in up to 10% of a flock and a 10%-15% fall in egg production. It occurs mainly in free-range hens or hens reared on the ground at around the time of peak lay. The incidence of the disease has increased in countries where there is an increase in keeping free-range laying hens. It is similar to the condition avian vibrionic hepatitis which was reported in America, Europe and Australasia in the 1950s to 1970s and the agent isolated from cases of avian vibrionic hepatitis and C.hepaticus appear to be very similar. It is not known if C.hepaticus is zoonotic but whole genome sequencing shows that it is most closely related to the known zoonotic campylobacters Campylobacter jejuni and Campylobacter coli. Human exposure to C.hepaticus is likely through similar exposure routes. Analysis of the whole genome showed a reduction in the genes for iron metabolism compared to C.jejuni. A requirement for iron was confirmed as it showed reduced growth in an iron depletion assay and this may explain its tissue tropism. With a move towards free-range egg production in many countries, the incidence of C.hepaticus hepatitis is likely to increase, but the identification of the causal agent will provide opportunities for the development of control methods.