Abstract
Japanese horseradish (Wasabia japonica Matsumura) is a perennial plant originated from Japan, but is currently grown in many other countries across the world. Wasabi contains important substances isothiocyanates (ITCs) which have potential positive influence on human health. Wasabi extract is also eventual a plant protection product. Therefore, using these positive features and obtaining an effective multiplication of this crop is an important element. This work focuses on methods of Wasabi propagation especially with the use of in vitro culture and the possible influence of the content of ITCs‑allyl isothiocyanate (AITC) on important substances in Wasabi.
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