A review of extraction and HPLC methods used for Melastomataceae and Asteraceae leaves in the analysis of flavonoids

Abstract

HPLC (high-performance liquid chromatography) is one of the accurate chromatographic techniques that is widely used for analysis. This present review submitted the extraction and HPLC methods for determining the compound in Melastomataceae and Asteraceae families that related with insecticidal compounds. This review discusses the standard used for the compound and chromatographic conditions. There are several species under both families. As for the Melastomataceae family, the species are Melastoma malabathricum and Miconia albicans meanwhile, the species under the Asteraceae family are Santolina semidentata, Eupatorium littorale, and Vernonia condensata. This review focus on the flavonoid and phenolic compound as these compounds are categorized as an insecticidal compound. HPLC can be used in conjunction with several detection technologies to identify flavonoids and phenolics in the plant, food, and biological samples because it is a method for separating these molecules. As a result, the HPLC procedure for the chosen molecule was reviewed in this article, which will aid in further analysis.