A Review of Challenges in Performing RT- PCR for COVID-19 in Rural Healthcare Settings

Aatmika Chauhan,Abhay Gaidhane,Anil Tambekar,Manoj Patil,Priti Karadbhajane,Shilpa Gaidhane,Zahiruddin Quazi Syed

doi:10.9734/jpri/2021/v33i60b34687

Abstract

COVID-19 pandemic has thoroughly shaken the community globally, including remote rural areas of all countries. Testing for SARS Cov-2 and clinical laboratory became the priority in hospital and healthcare settings during this pandemic. WHO confirmed the specificity and sensitivity of RT-PCR and announced the RT-PCR as the confirmatory test for the Covid-19 investigation. Governments mobilized all their vital resources towards establishing laboratories and enhancing the number of diagnostic tests to curb the spread of pandemics. In India's resource-limited limited settings, a molecular technique like RT-PCR faced several problems like improper laboratory setup, deficiency of trained health professionals, and many setbacks. This review reflects on challenges experienced in rural and resource-limited settings during the Covid-19 pandemic.

Highlights

IntroductionPCR is a technique that functions with either Deoxyribose nucleic acid (the genome) or ribonuclease acid (the transcriptome) of any living creature
PCR is a technique that functions with either Deoxyribose nucleic acid or ribonuclease acid of any living creature
The searching was done with the help of online databases

Summary

Introduction

PCR is a technique that functions with either Deoxyribose nucleic acid (the genome) or ribonuclease acid (the transcriptome) of any living creature. The genome can generate data that are instructive and informative for future studies [1]. The transcriptome is an mRNA complement wherein changes happen with the physiology, pathology, or development [1, 2]. This makes the data inside the transcriptome inherently versatile and variable. If this uncertainty is combined with the technological limitations of any reverse transcription (RT)-PCR assay, achieving a technically accurate and biological result becomes difficult [1]

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Discussion

Conclusion