Abstract
Chromosomal data on swamp buffalo especially in East Java province, is still lacking. For breeders, chromosomal analysis needs to be done to detect the possibility of hereditary genetic defects. In Malang Area, farmers lack of care and breeding programs for swamp buffalo also caused a decline in the genetic quality and performance. The purpose of this study was to compare the results of the chromosome analysis of swamp buffalo in Malang and another area in East Java province, which were selected based on differences in phenotypic characters. Chromosome analysis is very important for breeders because the results of this study can be used as a consideration for strategies to improve the genetic quality of swamp buffalo. The method used is standard karyotyping using whole blood with G banding staining. Chromosomal preparation using Karyo MAX medium, Colcemic Solution, Giemsa Stain, and KCl solution. Cell culture was performed according to the standard karyotyping method in mammals. The minimum number of 5 spreading chromosomes is the best was chosen, microphotographed, and then chromosome analysis is performed using Cytovision Image Analysis software. The results of this study did not find swamp buffalo with an abnormal number of chromosomes in East Java Province, Indonesia.
Highlights
Most of the livestock animals in Indonesia are categorized as a non-identified breed and practically have no sufficient genetic quality information, pedigree, lower productivity, and limited recording availability
Genetic analysis at the molecular and cellular level needs to be done through chromosome analysis, which can reportedly be used as a device for identifying and tracking animal origins [2]
The livestock used in this study were taken from several places the East Java region, where male buffaloes / bulls were found by showing phenotypic variations in coat and skin color, located in Malang district, Sumenep, and Kangean Island
Summary
Most of the livestock animals in Indonesia are categorized as a non-identified breed and practically have no sufficient genetic quality information, pedigree, lower productivity, and limited recording availability. Analysis of the history and origins of local Indonesian animals, especially local buffalo (swamp buffalo), are still compiled and described mainly based on information on phenotypic characters. This condition occurs because almost all of the buffalo are kept traditionally by small farmers in the countryside, not yet raised for commercial purposes. Genetic analysis at the molecular and cellular level needs to be done through chromosome analysis, which can reportedly be used as a device for identifying and tracking animal origins [2]
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