Abstract
Real-time quantification of glutathione (GSH) concentrations in living cells is essential for understanding GSH-related pathological events. However, most existing fluorescent probes are not suitable for this purpose due to their irreversible response mechanisms. Only a few probes have been successfully developed to realize this goal to date. Herein, we presented a fluorescent probe (QG-S) for quantifying cellular GSH and monitoring GSH dynamic variations. The probe QG-S was successfully employed in real-time monitoring of the dynamic variations of GSH in living cells upon reversible treatment with a GSH inhibitor (N-methylmaleimide) by using confocal microscopy. Moreover, the probe QG-S showed extremely low cytotoxicity and was facilely used to quantitatively determine the GSH concentration (4.25 mM) in living HeLa cells.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.