A Reversible Fluorescent Probe Based on a Redox-Switchable Excited-State Intramolecular Proton-Transfer Active Metal-Organic Framework for Detection and Imaging of Highly Reactive Oxygen Species in Live Cells.

Abstract

Detection and imaging of highly reactive oxygen species (hROS) in biological systems using fluorescent probes are critical for the study of physiological and pathological processes induced by hROS. Herein, we report a redox-active luminescent metal-organic framework (MOF), which incorporates a hydroquinone moiety that can undergo a reversible transformation from the hydroquinone to the quinone by hROS like •OH and ClO-. Moreover, the intrinsic fluorescence originating from the excited-state intramolecular proton transfer (ESIPT) property of the organic linker can be finely regulated during this redox-switchable process. A reversible fluorescent probe for hROS is thus developed. The presented probe shows a sensitive, selective, and reversible response to hROS due to the integration of excellent structural characteristics and unique spectral properties of the MOF. The detection limits of •OH and ClO- are 0.22 and 0.18 μM, respectively. Furthermore, with good photostability and super biocompatibility, this simple yet efficient fluorescent probe has been successfully applied to dynamic monitoring of endogenous and exogenous •OH and ClO- in live cells.