Abstract
A novel of Hg fluorescence probe (RS) with rhodamine chromophore was synthesized, and its structure was characterized by H NMR, MS spectra and elemental analysis. The recognition properties of the probe RS with Hg ions had been investigated in EtOH-HEPES buffer solution (V∶V=1∶1, pH=7.3) by the ultraviolet spectrophotometry and the fluorescence spectrophotometry. The results showed that the recognition is instantaneous and reversible. Upon the addition of Hg, the spirolactam ring of probe RS was opened and 1∶1 ligand-mercury complex was formed. The fluorescence imaging experiments of Hg in L929 living cells demonstrated that the probe RS could be used in biological systems.
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