Abstract
Abstract A reverse phase method for separating the four linear furanocoumarins psoralen, xanthotoxin, bergapten, and isopimpinellin in celery is described. The photosensitizing compounds, psoralen, xanthotoxin, and bergapten were also quantified in celery blades and leaves. The method utilizes a phenyl-bonded HPLC column with a methanol-water solvent system. This system allows the use of a detection wavelength (225 nm) more appropriate for these linear furanocoumarins than is currently used with normal phase systems.
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