A Reusable Column Method Using Glycopolymer-Functionalized Resins for Capture-Detection of Proteins and Escherichia coli.

Abstract

The use of glycopolymer-functionalized resins (Resin-Glc), as a solid support, incolumn mode for bacterial/protein capture and quantification is explored. The Resin-Glc is synthesizedfrom commercially available chloromethylated polystyreneresin and glycopolymer, and is characterized by fourier transform infrared spectroscopy, thermogravimetry, and elemental analysis. The percentage of glycopolymer functionalized on Resin-Glc is accounted to be 5 wt%. The ability of Resin-Glc to selectively capture lectin, Concanavalin A, over Peanut Agglutinin, reversibly, is demonstrated for six cycles of experiments. The bacterial sequestration study using SYBR (Synergy Brands, Inc.) Green I taggedEscherichia coli/Staphylococcus aureusreveals the ability of Resin-Glc to selectively captureE. colioverS. aureus. The quantification of captured cells in the column is carried out by enzymatic colorimetric assay using methylumbelliferyl glucuronideas the substrate. TheE. colicapture studies reveal a consistent capture efficiency of 105 CFU (Colony Forming Units) g-1 over six cycles. Studies with spiked tap water samples show satisfactory results forE. colicell densities ranging from 102 to 107 CFU mL-1 . The method portrayed can serve as a basis for the development of a reusable solid support in capture and detection of proteins and bacteria.