A retrospective study of clinical and laboratory characteristics of ovine footrot

Abstract

The infection of the feet of sheep by the anaerobic bacterium Dichelobacter nodosus results in a spectrum of diseases ranging from virulent through intermediate to benign footrot. By examining the clinical characteristics of various forms of footrot from 22 properties in Victoria, Australia in association with the results of several laboratory tests including the gene probe-based dot blot hybridisation, it was shown that virulent footrot could not be differentiated from intermediate footrot using the clinical characteristics alone. In addition, D. nodosus isolates from sheep with benign footrot as diagnosed by the clinical manifestations might in fact show characteristics associated with those causing virulent, intermediate or benign footrot as determined by the laboratory tests. Although the elastase test, gelatin gel test and dot blot were in agreement with isolates from 9 of the 22 properties under investigation, they displayed differences from one another in many other cases, especially those with clinical benign footrot. The results of the elastase test appeared to be slightly closer to those of the dot blot compared with the gelatin gel test. Like any other tests based on the measurements of phenotypic characteristics of D. nodosus bacteria, the elastase test and gelatin gel test are subject to changes with factors that affect bacterial growth and metabolism. The use of DNA based detection methods would eliminate many of the problems associated with the conventional tests for differentiating strains causing virulent, intermediate and benign footrot.