Abstract
A retrospective study of the clonal structure of 24 Vibrio cholerae eltor strains isolated during epidemic complications in the Russian Far East was carried out on the basis of a macrorestrictive analysis of Vibrio cholerae eltor genomic DNA using NotI and SfiI endonucleases. It was found that clonal (by amplification profiling data) toxigenic strains (n = 23) were characterized by variability of the NotI/SfiI-generated restriction profiles. The V. cholerae eltor isolated during an outbreak in the city of Yuzhno-Sakhalinsk were differentiated into four pulse types, with one dominating type detected in 76.5% of the strains. A separate NotI/SfiI-restriction profile was characteristic for the isolates from Primorskii krai. A nontoxigenic strain formed a separate dendrogram line from the toxigenic strains on the basis of the PFGE-pattern structure. The use of macrorestriction analysis was shown to be effective for deep characterization of the V. cholerae population structure and for ascertainment of molecular-epidemiological regularities in the territorial distribution of certain V. cholerae clones.
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