Abstract
Multi-spectral imaging (MSI) has made diagnosis of microscopic samples considerably easier and information abound. Most MSI systems use continuum light sources and filters for imaging purposes. However, these light sources and filters are relatively expensive, unstable due to extreme pressure and temperature and associated with prolong acquisition time. In this work, we present a metallurgical microscope retrofitted with light-emitting diodes (LEDs) as illumination sources for MSI microscopy. This multispectral LED imaging microscope (MSLEDIM) is relatively cheaper and capable of acquiring images in reflection, transmission and scattering modes at thirteen (13) different wavelengths ranging from ultraviolet to near infrared. The microscope has been demonstrated in biomedical and entomological research fields. The MSLEDIM can be used in various scientific research fields for imaging microscopic samples.
Highlights
Multi-spectral imaging (MSI) has been suggested as a non-destructive method used in many applications to quantify biological processes (Hu et al, 2005; Frey & Warda, 2008; Teikari, 2008; Bautista & Yagi, 2011; Cosentino, 2015)
We present a retrofitted metallurgical microscope which uses nine (9) light-emitting diodes (LEDs) emitting a total of 13 wavelengths ranging from 375 nm to 940 nm
Light sources used in MSI microscopy have restricted number of spectral bands associated with comparatively long acquisition times
Summary
Multi-spectral imaging (MSI) has been suggested as a non-destructive method used in many applications to quantify biological processes (Hu et al, 2005; Frey & Warda, 2008; Teikari, 2008; Bautista & Yagi, 2011; Cosentino, 2015). During MSI application in microscopy, the selective transmission of light with different wavelengths by the filters depends on the stability of the spectrum of light source used and the time required during acquisition. The light sources have limited number of spectral bands with relatively long acquisition time This introduces uncertainties which lead to sample photo-bleaching and photo-kinetics, which may cause non-linear absorption and fluorescence (Brydegaard et al, 2011). We present a retrofitted metallurgical microscope which uses nine (9) LEDs emitting a total of 13 wavelengths ranging from 375 nm to 940 nm These are used as illumination light sources for MSI in reflection, transmission and scattering modes
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