Abstract

A 20-channel Gilson differential respirometer has been modified to evaluate potential physiological adjustments of small aquatic animals exposed to rapid thermal changes. Time-temperature conditions can be achieved which are typical of large volume steam electric generating stations with once-through cooling systems terminating in submerged discharges. In entrainment studies the modified respirometer has been used for determining pre-stress oxygen consumption, simulation of a time-temperature curve, followed by subsequent post-stress oxygen consumption data. All reaction flasks remain connected to the volumometers throughout the entire operation, thereby avoiding the necessity of removing and resealing the reaction flasks to the connectors. This also eliminates the problem of transferring organisms in and out of the reaction flasks during a study.

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