A research on the pathological mechanisms of aortic dissection

Abstract

In this paper, the main content is to investigate which experimental methods are needed in the study of the case mechanism of aortic dissection and how to analyze the results by analyzing the experimental methods. The researchers used RNA sequencing, ChIP-Seq technology, transcription factor prediction, the dual-luciferase reporter assay, immunohistochemical staining, immunofluorescent staining, matrix metalloproteinases (MMP) activity staining, western blot and statistical analysis. The researchers used quantitative RT-PCR, In-Gel zymography, oxidant measurement by dihydroethidium, and flow cytometric analysis of inflammatory cells.