A renewable platform based on the entropy-driven catalytic amplification and element labeling inductively coupled plasma mass spectrometry for microRNA analysis.

Abstract

The element labeling inductively coupled plasma mass spectrometry (ICP-MS) strategy has been increasingly applied to the bioanalysis for various bio-targets. Herein, a renewable analysis platform with element labeling ICP-MS was firstly proposed for microRNA (miRNA) analysis. The analysis platform was established on the magnetic bead (MB) with entropy-driven catalytic (EDC) amplification. When the EDC reaction was initiated by target miRNA, numerous strands labeled with Ho element were released from MBs, and 165Ho in the supernatant detected by ICP-MS could reflect the amount of target miRNA. After detection, the platform was easily regenerated by adding strands to reassemble EDC complex on MBs. This MB platform could be used four times, and the limit of detection for miRNA-155 was 8.4pmolL-1. Moreover, the developed regeneration strategy based on EDC reaction can be easily expanded to other renewable analysis platforms, such as, the renewable platform involving EDC and rolling circle amplification technology. Overall, this work proposed a novel regenerated bioanalysis strategy to reduce the consumption of reagent and time for probe preparation, profiting the development of bioassay based on element labeling ICP-MS strategy.