Abstract
A rapid, convenient and accurate method using a sol–gel–alginate–carbon composite electrode (SACE) is described for the determination of β-indole acetic acid (IAA) in plant samples. The determination is based on an enzyme-linked competitive immunoreaction between free IAA and IAA labeled with HRP to bind on the anti-IAA IgG immobilized on the SACE surface. The response signal expressed as percentage current reduction (CR%, y) is linearly related to the logarithm of the concentration of IAA ( x) in range of 5–500 μg ml −1 with a regression equation of the form y=37.80 x−22.47 and correlation coefficient of 0.9922. The immunosensor was used to determine the IAA content in the hybrid rice grain samples taken in the growing period.
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