Abstract
The objectives of this study were to determine (1) if levels of 8-isoprostaglandin F2α (8-isoPGF2α), a non-enzymatically produced prostaglandin, were increased after cortical contusion and (2) if enzyme immunoassay (EIA) could be used to quantify 8-isoPGF2α levels. 24 h after the contusion there was a significant rise in 8-isoPGF2α compared to control levels. The levels returned to baseline by 72 h postinjury. The results show that this EIA method is a reliable and sensitive technique for measuring brain lipid peroxidation.
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