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Abstract
Various methods of steroid analysis were assessed using radiolabeled steroids and thin layer chromatography. Three reversed phase chromatography systems were evaluated for separation and recovery of steroids extracted from blood, liver tissue and feces. The use of different numbers of Sep-Pak C(18) cartridges for the purification of steroid extracts was examined and steroid recoveries were measured and compared. The results indicated that recoveries were best when 4-6 cartridges were used. Rapid and slow procedures of enzymatic hydrolysis and acidic solvolysis of steroid conjugates were compared. A new and relatively rapid method for analysis of steroid profiles in liver, blood and fecal samples was developed. Assessment of this method showed that steroid recoveries were improved compared to existing methods with percentage recoveries of 64.1-82.5 for liver samples, 55.2-75 for blood samples, and 65.1-76.3 for fecal samples.
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