Abstract
A 2028-bp fragment from the RF DNA of φLf, a filamentous phage ofXanthomonas campestrispv.campestris,was maintained autonomously as a minireplicon. Upon superinfection of the cells harboring the minireplicon with φLf, transducing miniphage particles were released. The minireplicon contained an open reading frame (ORF346) able to encode a polypeptide of MW 39144, which possessed consensus motifs found in the Rep proteins from various sources. These findings suggested ORF346 to be the gene encoding replication initiation protein, gene II (gII) of φLf. Upstream to ORF346 were sequences with potential to form hairpin structures and a sequence similar to the integration host factor (IHF) binding site, structures similar to the intergenic region (IR) of the Ff phages. A 15 bp AT-rich core for φLf integration was found 37 bp downstream to the IHF binding site.
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