Abstract
The reliable taxonomic identification of organisms through DNA sequence data requires a well parameterized library of curated reference sequences. However, it is estimated that just 15% of described animal species are represented in public sequence repositories. To begin to address this deficiency, we provide DNA barcodes for 1,500,003 animal specimens collected from 23 terrestrial and aquatic ecozones at sites across Canada, a nation that comprises 7% of the planet’s land surface. In total, 14 phyla, 43 classes, 163 orders, 1123 families, 6186 genera, and 64,264 Barcode Index Numbers (BINs; a proxy for species) are represented. Species-level taxonomy was available for 38% of the specimens, but higher proportions were assigned to a genus (69.5%) and a family (99.9%). Voucher specimens and DNA extracts are archived at the Centre for Biodiversity Genomics where they are available for further research. The corresponding sequence and taxonomic data can be accessed through the Barcode of Life Data System, GenBank, the Global Biodiversity Information Facility, and the Global Genome Biodiversity Network Data Portal.
Highlights
Background & SummaryHigh-throughput sequencing platforms have enabled a novel approach to biodiversity surveys by making it possible to identify the array of species present in bulk collections or environmental DNA1–3
The retention of DNA extracts provides a basis for ‘upgrading’ records if the barcode standard eventually adopts new approaches in groups, such as plants, where data standards have changed[34], or for sequencing the entire genome[35]. This data resource presents a curated DNA barcode reference library for a sizeable fraction of the Canadian invertebrate fauna, illustrates the workflows involved in its construction, and describes the resources resulting from this effort
The curated DNA barcode reference library presented here for Canadian invertebrates was constructed by a series of workflows that generate diverse products and accessible resources (Fig. 1)
Summary
High-throughput sequencing platforms have enabled a novel approach to biodiversity surveys by making it possible to identify the array of species present in bulk collections or environmental DNA1–3. The retention of DNA extracts provides a basis for ‘upgrading’ records if the barcode standard eventually adopts new approaches in groups, such as plants, where data standards have changed[34], or for sequencing the entire genome[35]. This data resource presents a curated DNA barcode reference library for a sizeable fraction of the Canadian invertebrate fauna, illustrates the workflows involved in its construction, and describes the resources resulting from this effort. We anticipate it will have wide utility in metabarcoding and related studies, due to the diversity of taxa included
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