Abstract
Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Here we report a reference-grade genome of wild soybean accession W05, with a final assembled genome size of 1013.2 Mb and a contig N50 of 3.3 Mb. The analytical power of the W05 genome is demonstrated by several examples. First, we identify an inversion at the locus determining seed coat color during domestication. Second, a translocation event between chromosomes 11 and 13 of some genotypes is shown to interfere with the assignment of QTLs. Third, we find a region containing copy number variations of the Kunitz trypsin inhibitor (KTI) genes. Such findings illustrate the power of this assembly in the analysis of large structural variations in soybean germplasm collections. The wild soybean genome assembly has wide applications in comparative genomic and evolutionary studies, as well as in crop breeding and improvement programs.
Highlights
Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources
To investigate a more complex case, we examined the I locus on chromosome 8, which determines the pigmentation of the seed coat[23], a major trait that was selected during domestication[21]
The availability of multiple high-quality reference genomes from diverse genetic backgrounds is a prerequisite for effective mining of crop genomes[30], especially in studies involving wild germplasm
Summary
Efficient crop improvement depends on the application of accurate genetic information contained in diverse germplasm resources. Accurate information is required for effective tracking of genomic variations, mapping important quantitative trait loci (QTLs), and discovering novel alleles. These tasks are intrinsically dependent on the availability of a range of genetic resources and, most crucially, high-quality reference genomes[1]. We demonstrate the power of W05 reference genome and highlight its applicatibity in a wide range of comparative genomic and evolutionary studies, using a range of examples, such as the identification of large structural variations, QTLs, genes, and alleles. The advantages of combining high-quality reference genomes and optical mapping (OM) in studying structural variations among multiple accessions is described
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
