Abstract
A flow-through test system was designed to examine minimum dissolved oxygen (D.O.) requirements of marine animals. The system provides up to six treatment concentrations between 0.3 mg D.O./l and saturation. Sea water is degassed in a vacuum-evacuated packed column and the treatment concentrations are controlled electronically by time-proportional mixing of water from degassed and air-saturated seawater reservoirs. The system has proven capable of reliable continuous operation for test periods as long as 28 days, with the reduced D.O. treatments controlled to ±0.15 mg/l s.d. at flows ≥10 ml/min and to ±0.25 mg/l s.d. at lower flow rates.
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