Abstract

As important members of biothiol species, cysteine (Cys), homocysteine (Hcy) and glutathione (GSH) are involved in numerous physiological activities. Moreover, abnormal levels of these biothiols are closely relevant to a series of diseases. Therefore, it's of great importance and challenges to develop efficient approaches for sensitively detecting biothiols. Herein, a novel fluorescent probe JSC-RSH for sensing Cys/Hcy/GSH was reported. JSC-RSH exhibits excellent properties including red-emitting, high sensitivity and selectivity, as well as rapid response towards biothiols. The probe alone only emits negligible fluorescence, while a remarkable fluorescence emission in the red region could be observed in the presence of biothiols. The calculated limits of detection (LODs) for Cys/Hcy/GSH are 7.9 nM, 10.2 nM and 4.2 nM, respectively. Furthermore, probe JSC-RSH with low cytotoxicity was utilized for monitoring endogenous and exogenous biothiols in A549 cells.

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