Abstract
Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis transmitted via the fecal–oral route. Both viruses place a heavy burden on the public health and economy of developing countries. To test the possibility that HAV could be used as an expression vector for the development of a combination vaccine against hepatitis A and E infections, recombinant HAV-HEp148 was created as a vector to express an HEV neutralization epitope (HEp148) located at aa 459–606 of the HEV capsid protein. The recombinant virus expressed the HEp148 protein in a partially dimerized state in HAV-susceptible cells. Immunization with the HAV-HEp148 virus induced a strong HAV- and HEV-specific immune response in mice. Thus, the present study demonstrates a novel approach to the development of a combined hepatitis A and E vaccine.
Highlights
Hepatitis A virus (HAV) and hepatitis E virus (HEV) are causative agents of acute viral hepatitis.HAV is a small, non-enveloped virus with a positive-sense RNA in the family Picornaviridae [1].HAV infection is responsible for approximately 1.4 million new cases worldwide each year
HAV titers were determined by inoculating the confluent monolayer of cells cultured in 25 cm3 culture flasks, and infection was verified by a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA)
The sequencing results showed that the 444 nts sequence was inserted into the polylinker at the 2A/2B junction of pT7-HAV, as expected, and that the reading frame was correct
Summary
Xiang Kui 1,2 , Kusov Yuri 3,4 , Ying Guan 5 , Yan Wang 2 , Shan Yi 1 , Jinyuan Wu 1 , Na Yin 1 , Yan Zhou 1 , Hongjun Li 1, * and Maosheng Sun 1, *. German Center for Infection Research (DZIF), Hamburg-Lübeck-Borstel-Riems Site, University of Lübeck, 23562 Lübeck, Germany. Received: 22 July 2017; Accepted: 9 September 2017; Published: 15 September 2017
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