Abstract
Azoxystrobin-resistant populations of Alternaria spp. in the alternata, tenuissima, and arborescens species–groups, the causal agents of Alternaria late blight of pistachio, have been selected in pistachio orchards in California. The azoxystrobin resistance in Alternaria spp. was found to be correlative to a single point mutation resulting in the replacement of a glycine by an alanine at codon 143 (G143A) in the mitochondrial cytochrome b ( cyt b) gene. Based on this point mutation, a real-time PCR method was developed to rapidly detect azoxystrobin-resistant Alternaria populations in field leaf samples collected from pistachio orchards in California.
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