A Real-Time PCR Assay to Detect Human Cytomegalovirus DNA in Amniotic Fluid of Primarily Infected Women

Abstract

(P = 0.32).Women who had coexisting medical conditions like Diabetes and Sickle cell disease (P = 0.0019) and those who had HIV positive husbands (P = 0.0476) had statistically higher prevalence of AB. Age, religion, previous UTI, coital frequency, parity and ethnic group did not confer statistical significance. Among the HIV positive women, other parameters were evaluated. The CD4 count, viral loads, antiretroviral drug use, duration and compliance and duration of positivity were not contributory its development. There was no statistical significance between the causative organisms among the negatives and positives and constituted mainly uropathogens.Most organisms cultured were resistant to common antibiotics like Gentamycin and nitrofurantoin in both negatives and positives alike. The organisms were sensitive to cephalosporin. In the women found to have asymptomatic bacteriuria, there was no significant development of anaemia, preterm delivery, low birth weight and development of pre eclampsia when followed up to delivery. These had previously been reported by other workers. Conclusion: There is no statistically significant association between the developments of AB among HIV infected and uninfected women in pregnancy. Routine screening should be offered to those with coexistent medical disorders if not the entire antenatal populace. Antibiotic use should be cautious to halt resistance.