Abstract
A label-free electrochemical aptasensor was fabricated for reagentless determination of tetracycline (TET) in water. A 5′-amino-modified 76-mer oligonucleotide was immobilized on screen-printed carbon electrode via carbodimide coupling. Cyclic voltammetry experiments revealed an unexpected electroactivity of immobilized aptamer, showing a quasi-reversible signal with oxidation and reduction peaks at respectively +0.19 and −0.25 V vs Ag/AgCl. The binding of TET on the aptasensor induced a decrease of oxidation peak, allowing the detection of TET for concentrations ranging from 0.05 μg/L to 20 μg/L, with a detection limit of 0.035 μg/L. The proposed aptasensor displayed a good specificity for TET and was successfully used for the detection of TET in natural waters, with recovery rates ranging from 87.8 to 97%.
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