Abstract

We examined the stability of retinol in blood and serum samples, kept in the dark, under different handling procedures. Samples not protected from contact with air oxygen were highly unstable, even when kept at ice temperature. Samples collected under anaerobic conditions, with Vacutainer Tubes, or treated with nitrogen after collection to displace the air from the tubes were stable during the usual interval between collection and freezing or analysis in biochemical surveys. Ingestion of a moderate amount of vitamin. A significantly increased serum retinol concentrations in normal volunteers, showing the importance that survey samples be preprandial.

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