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Abstract
Double-label immunocytochemistry was utilized to re-examine the colocalization of enkephalin and γ-aminobutyric acid (GABA) in amacrine cells of the larval tiger salamander retina. A total of 465 enkephalin-immunoreactive amacrine cells were identified and in all cases these cells were GABA-immunoreactive. This finding corroborates a previous study that showed greater than 96% of enkephalin-amacrine cells in the tiger salamander retina to specifically accumulate [ 3H]GABA and provides additional evidence for the GABAergic nature of these enkephalin-amacrine cells.
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