Abstract
A novel ratiometric fluorescent probe for the detection of γ-glutamyl transpeptidase (GGT) activity was developed. The recognition of GGT by this probe is achieved by its enzymatic hydrolysis of peptide bonds in the probe. Under optimized conditions, the probe exhibited obvious ratiometric fluorescence responsiveness after interaction with GGT, avoiding potential background interference. The linear concentration range was 0.0350–2 U / mL, with good selectivity and stability. Under a 365 nm UV lamp, as the concentration of GGT increased, the probe aqueous solution gradually changed from dark blue to light blue. In addition, the B/G value of the probe's solution and the GGT activity were linearly correlated. More significantly, the probe was able to effectively identify the GGT activity of garlic.
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