A ratiometric fluorescence probe for the selective detection of H2S in serum using a pyrene-DPA-Cd2+ complex.

Jihoon Kim,Min Su Han,Jinyoung Oh

doi:10.1039/d1ra04277g

Jihoon Kim, Min Su Han + Show 1 more

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https://doi.org/10.1039/d1ra04277g

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Abstract

A ratiometric and selective hydrogen sulfide (H2S) detection probe was proposed based on the pyrene-DPA–Cd2+ complex through the metal ion displacement approach (MDA) mechanism. While most MDA-based fluorescence probes with paramagnetic Cu2+ have focused on the development of a simple turn-on sensor using the broad spectral range of fluorescence enhancement, this ratiometric probe exhibited unchanged monomer emission as a built-in internal reference with an increase in excimer emission with added H2S. The demonstrated probe showed a rapid response (within 1 min) and a high sensitivity, with 70 nM as the limit of detection. The selectivity for H2S over cysteine, homocysteine and glutathione was confirmed, and reliable fluorescence enhancement, which could be monitored by the naked eye, was observed upon irradiation with handheld UV light. In addition, this detection system was successfully applied to detect H2S in human serum without interference from biological molecules.

Highlights

The pyrene-DPA–Cd2+ complex could be used as a ratiometric probe for selective H2S detection over biothiols with high sensitivity and applicability in real serum samples, which were derived from the features of the metal ion displacement approach (MDA) mechanism
Considering that the pyreneDPA–Zn2+ complex was previously used as a probe for the detection of PPi, which is abundant in a biological environment, uorescence changes under interference from PPi were measured (Fig. S2†).[32]
Unlike the pyrene-DPA–Cd2+ complex, the pyrene-DPA–Zn2+ complex displayed an increase in excimer emission in the presence of PPi

Summary

Introduction

Hydrogen sul de (H2S) is a gaseous signaling molecule that has received attention because of its toxicity for physiological functions in humans.[1,2] Endogenous H2S, which is generated mainly from cysteine (Cys), homocysteine (Hcy), and 3-mercaptopyruvate by enzymatic reactions of cystathionine b-synthase, cystathioine-g-lyase, and 3-mercaptopyruvate sufurtransferase/cysteine aminotransferase, is involved in various biological processes such as blood pressure regulation, metabolic disorders, neurodegeneration, and in ammation.[3,4,5,6,7,8] the imbalanced synthesis of endogenous H2S is related to some human diseases such as lung infection, Alzheimer's disease, and diabetes.[9,10,11] abnormal levels of endogenous H2S in human serum can be used as evidence for such diseases. The pyrene-DPA–Cd2+ complex was proposed for use in a ratiometric uorescence probe for H2S detection that is selective to other biothiols (Scheme 1).

Results

Conclusion