Abstract
Abstract A coumarin-quinoline based fluorescence resonance energy transfer (FRET) system ( TCQ ) has been synthesized and employed as a ratiometric fluorescence probe. The selective fluorescent response of the probe TCQ toward Al 3+ was devised by employing a quinoline moiety as a FRET energy donor with a coumarin moiety as an energy acceptor. The quinoline emission at 390 nm decreased and the coumarin emission at 480 nm increased concurrently on addition of Al 3+ under excitation wavelength at 253 nm. The TCQ probe exhibited high selectivity for Al 3+ as compared to other tested metal ions and the ratiometric sensing of Al 3+ was determined by plotting the fluorescence intensity ratio at 480 nm and 390 nm versus Al 3+ ion concentration. Moreover, test strips based on TCQ were fabricated, which were found to act as a convenient and efficient Al 3+ ion detection kit. Furthermore, this system has been used for imaging of Al 3+ in living cells.
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