Abstract
A new single-point calibration immunoprecipitation inhibition rate nephelometric assay for phenobarbital (PB) and phenytoin (PT) has been evaluated on the Beckman ICS-II analyzer. Within-run precision (n = 20) coefficients of variation (CV) were less than 4.0% for PB (10-44 micrograms/ml) and less than 5.7% for PT (12-34 micrograms/ml). Between-run precision CVs of pooled sera (n = 20) assay were less than 4.2% for PB (7.5-33.8 micrograms/ml) and less than 8.7% for PT (4.5-17.8 micrograms/ml). Linearity and recoveries were good over a broad range of drug levels for both assays. Excellent correlation was found with existing PB, ICS vs. EMIT on the IL Multistat III MCA (y = 1.26 + 1.00x; r = 0.994), ICS vs. Dupont aca (y = 0.832 + 1.01x; r = 0.997), and ICS vs. GLC (y = 0.08 + 0.99x; r = 0.998) methods, and PT, ICS vs. MCA (y = -1.76 + 1.04x; r = 0.985), ICS vs. aca (y = -2.02 + 1.054x; r = 0.987), and ICS vs. GLC (y = -0.26 + 0.990x; r = 0.999) methods. These assays were found to provide accurate drug analyses, good stat or batching capability, and good correlation with existing methods.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
